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However, Virgil van Dijk's header in the second minute of added time clinched the victory for Liverpool, leading to Atletico manager Diego Simeone being sent off.Read More.
Alexander Isak finally made his debut for the Reds following his £125 million ($170.5 million) transfer deadline day switch from Newcastle earlier this month.
The Swedish international had only played 18 minutes since May, coming on for his national side in a 2-0 loss to Kosovo during the international break.
After ramping up his fitness training last week at the AXA Training Centre, he was included in Slot's starting line-up against Simeone's team.
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Curtis Jones remains an injury concern for Liverpool.
The 24-year-old missed the journey to Burnley after picking up a knock against Arsenal prior to the international break.
There's no set date for his comeback, but the injury is not thought to be severe, and there's optimism he could be back in action for the match against Everton.
"Curtis will train with the team and hopefully able to be in the squad on Saturday, but not for tomorrow (against Atletico)," Slot stated before Wednesday's game.
The Liverpool boss also spoke to TNT Sports after beating Atletico
"If you want to beat a team like Atletico, which has an unbelievable mentality, you have to beat them with their weapon as well," said Slot.
"That is what we showed again today - that we can beat them with mentality
"But again we showed mentality because in that 10 minutes that still had to be played, we were pushing, pushing, pushing, and we scored a set-piece goal."