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Huge crowds are expected at the service in the footballer’s hometown of Gondomar near Porto.
The father of three, who had recently married his long-term partner Rute Cardoso, was killed alongside his brother Andre Silva after their car burst into flames following a suspected tyre blowout in the early hours of Thursday.
The funeral, which begins at 10am, follows a wake held at the Capela da Ressurreicao yesterday.
The brothers’ parents attended the Sao Cosme Chapel, with Portuguese President Marcelo Rebelo de Sousa, Prime Minister Luis Montenegro and Jota’s agent also in attendance.
Also present were Liverpool executives and backroom staff, including CEO Billy Hogan, technical director Julian Ward - a former Larne player - and sporting director Richard Hughes.
Queues formed outside the church as people held service sheets featuring pictures of both brothers, the largest one showing Jota smiling in his Liverpool shirt and making a heart sign with his hands.
Liverpool has postponed the players’ return for pre-season training following Jota’s death.
Manager Arne Slot said the “sense of shock is absolute”, adding: “He was a loved one to all of us.”
On Friday former Liverpool captain Jordan Henderson looked visibly upset as he returned to Anfield with flowers and a club scarf.
A sea of floral tributes has been left outside the ground.
The club’s Northern Ireland player Conor Bradley said Jota was "an incredible footballer but an even better person”.
“When I first moved up from the academy you were always there for me and always helped me settle in with the lads which I’ll be forever grateful for,” he said.
“My thoughts are with your wife, children and family for losing both Diogo and Andre.
I can’t imagine how tough this is for you all, as I know how tough this feels for me today.
“Love you Jots, and I’m sure we will meet again.”
In Belfast, Liverpool supporters gathered to lay flowers outside the club’s city centre merchandise store.
The store was closed on Friday as a mark of respect, with cards and flowers laid in his honour.